Bland-Altman plots of the first-second response differences for nil, mitogen, and TB were presented in Fig. . TB test results in kidney transplant candidates Now going through your report carefully, it shows that the report is positive. M. tuberculosis complex infection unlikely. Epub 2013/03/23. mitosis : a substance that induces mitosis. Specimen Requirements. Pokeweed Mitogen (PWM)-induced Lymphocyte Proliferation Measurement of human lymphocytes proliferative responses to various stimuli is a fundamental technique used to assess their biological status and functions. Predictive value of interferon-gamma release assays for incident active tuberculosis: a systematic review and meta-analysis. Test Name Results Normal Range Units Report Status: FINAL . Procentrioles elongate until they reach the length of the parent centrioles in late G2. doi: Rangaka MX, Wilkinson KA, Glynn JR, Ling D, Menzies D, Mwansa-Kambafwile J, et al. You have been denied access to this content. QFT-PLUS Result Report/Interpretation 8.0. Quantiferon TB minus NIL: result 0.40 H, ref ranges 0.00-0.35 IU/mL. Clients with questions concerning how to order the QuantiFERON - TB Gold PLUS tubes can call Client Services at 800-628-6816 or 216-444-5755. . . Contact Microbiology if additional instructions or information is needed. Store blood collection tubes at 4C to 25C. QIAGEN Inc. Tel: +1.661.775.7480 www.quantiferon.com 27220 Turnberry Lane, Suite 200 Fax: +1.661.775.7479 www.qiagen.com Valencia, CA 91355 September 23, 2013 Re: Customer reports of higher than expected rates of mitogen indeterminate results using the QuantiFERON -TB Gold (QFT) test. The agents which stimulate cell division are called mitogens, e.g., cytokinins, some steroids, however, some chemicals inhibit cell division, these are called mitotic poisons, e.g., azides, cyanides, colchicine, chalones, Page 2 etc. The QuantiFERON -TB Gold IT system uses specialized blood collection tubes, which are used to collect whole blood via venipuncture, which include a Nil control tube, TB Antigen tube and a Mitogen tube (positive control). TB response 0.25-0.35 IU/ml, regardless of nil control value. Microbiol Spectr. DO NOT REFRIGERATE OR FREEZE. These pathways control gene expression in a number of ways including the phosphorylation and regulation of transcription factors, co-regulatory proteins and chromatin proteins. Compare Search ( Please select at least 2 keywords ) Most Searched Keywords. 2014 Oct;11(8):1267-76. doi: 10.1513/AnnalsATS.201405-188OC. Quantiferon gold mitogen nil. Depending on the strength of the immune response, a medical provider can determine if there is the possibility of an infection. Samples collected on Saturday or Sunday must be received at Cleveland Clinic Laboratories by 11:00 p.m. See Special Information section. Quantiferon-TB Gold In-Tube Test (10010388) Test Mnemonic: QUANTIFERON-TB GOLD. It is commonly seen in prokaryotes. After incubation, mark the tubes as "INCUBATED". TB2 Ag minus Nil Result: 88517-8: DEXQG: Mitogen minus Nil Result: 71774-4: DEXQH: Nil Result: 71776-9: . This indicates that the test is functioning properly. The "normal," "weak" and "negative" mitogen response (respectively) was associated with decreasing hemoglobin, albumin and lymphocyte counts, but increasing neutrophil count and C-reactive protein levels ( Table 1 ). Before In cases of hypercalcemia with suppressed PTH and no known active malignancy, . Cited above, the TB antigen tube value minus the Nil tube value is within 29 the InBios active TbDetectanti-TB IgG antibody assay we examined 100 patients 34! What is a normal mitogen level? If unable to contact Virology and uncertain how to proceed, call Lab Medicine Resident at 206-598-6190. Clipboard, Search History, and several other advanced features are temporarily unavailable. QFT is the most clinically tested and proven IGRA. If the level of blood in any tube is outside of the indicator mark, a new blood sample should be obtained. Tubes on the Supply Storefront available at our website www.clevelandcliniclabs.com DoctorSpring < /a > Nil tube value is well the. My thesis aimed to study dynamic agrivoltaic systems, in my case in arboriculture. Some facilities use a broader Borderline range and suggest having these patients retested in 4-8 weeks. . . A systematic review. A chest radiograph showed almost complete resolution of airspace disease. Collection Instructions: 1. Any substance that triggers mitosis, as well as lymphocyte blastogenesis. In follow-up samples, individuals with initial results between 0.20-0.34 IU/ml and 0.35-0.99 IU/ml displayed negative results below the borderline range (<0.20 IU/ml) in 66.1% (230/348) and 42.5% (285/671) respectively, and none developed incident TB. A positive TB blood test result means you probably have TB germs in your body. Value is well above the minimum breakpoint of 0.5 IU/ml known active malignancy, positive the. Epub 2021 Nov 10. The laboratory performing the assay participates in an . : Mitogen minus Nil result: 71776-9: TB Ag, INTERFERON GAMMA results positive TB! results cannot be interpreted as a result of low mitogen response or high background response. doi: Welfare TSNBfHa. Quantiferon nil value. Mitogen minus Nil (IU/ml) QFT-Plus result Report/Interpretation < 8.0 0.35 and 25% of Nil Any Any Positive M. tuberculosis infection likely Any <0.35 or . Using plastic pipette, add whole blood from the Lithium Heparin tube to the fill line on each of the tubes QTB Tubes. Document incubation time and temperature on the requisition. A result was considered indeterminate if: (1) the blood sample did not respond appropriately to either the positive or negative tube and, thus, could not be interpreted, or (2) the concentration of IFN- in the antigen well minus that in the nil well was <0.35 IU/mL but the concentration of the cytokine in the mitogen well was <0.5 IU/mL or (3 . Copyright and Disclaimer, Department of Laboratory Medicine & Pathology. MMWR Recommendations and reports: Morbidity and mortality weekly report Recommendations and reports. Cleveland Clinic Laboratories. Specimen Requirements: Collection: Collect 5 mL of whole blood into a 6 mL blood collection tube containing Lithium-Heparin as the anticoagulant. 2013;31:475527. n tested. Mitogen minus Nil Result; TB Interpretation; Performing Laboratory. The Mitogen minus Nil value serves as the positive control, demonstrating the successful responsiveness of the T lymphocytes in . TB1-Nil >0.35 is The tubes are shaken to mix antigen with the whole blood and incubated at 37 C + 1 C for 16 to 24 hours. Epub 2021 Sep 22. QuantiFERON-TB Gold Plus (QFT-Plus) . M. tuberculosis complex infection unlikely. Reference Ranges. As 1 mL tubes draw blood relatively slowly, keep the tube on the needle for 2 - 3 seconds once the tube appears to have completed filling. University of Nevada, Reno | School of Medicine | School of Medicine . Finding ( TB antigen tube value must be 0.35 IU/ml reliable technology that you trust tube! Mix antigen with the whole blood DIRECTLY into each tube of the complete blood i.e. QuantiFERON-TB Gold Plus is an in vitro diagnostic test using a peptide cocktail of CD4+ and CD8+ T cell antigens to stimulate cells in heparinized whole blood. 27 QuantiFERON-TB Gold In-Tube (QFT-GIT) assay was conducted at University of Utah, 28 ARUP Laboratories, November 2008 to December 2010. FOIA Confirm volume in all 4 tubes (Grey top NIL tube, Green top TB1 ANTIGEN tube, Yellow top TB2 ANTIGEN tube and Purple top MITOGEN tube) is at or near the black fill mark. Quantiferon-TB Gold Plus Description This test is for the diagnosis of latent TB (tuberculosis) infection, but not of active TB disease which requires AFB culture and smear (See AFBHSC). Quantiferon Mitogen minus NIL: result > 10.00 IU/mL. A 30 positive QFT-GIT, 59 ( 5.7 % ) were positive for M. tuberculosis antibodies all. Epub 2011/08/19. A mitogen is a small bioactive protein or peptide that induces a cell to begin cell division, or enhances the rate of division (). Each daughter cell inherits two centrioles (one centrosome) surrounded by pericentriolar material as a result of cell division. QuantiFERON-TB Gold In-Tube Test is an in vitro laboratory diagnostic test which is used as an aid in diagnosing Mycobacterium tuberculosi s infection or disease. In absence of any symptoms, it seems to be positive, the TB antigen Nil! NIL 0.01 IU/mL MITOGEN-NIL >10.00 IU/mL TB1-NIL 0.00 IU/mL TB2-NIL 0.01 IU/mL The Nil tube value reflects the background interferon gamma immune response of the patient's . IU/mL. Many mitogens are lectins, carbohydrate-binding proteins usually derived from bacteria or plants. Request QuantiFERON - TB Gold PLUS tubes on the Supply Storefront available at our website www.clevelandcliniclabs.com. QuantiFERON MITOGEN minus NIL: No reference interval . Fig 1. eCollection 2022 Dec 22. Test Name Result Flag Reference Range Lab QUANTIFERON(R)-TB GOLD QUANTIFERON(R)-TB GOLD NEGATIVE NEGATIVE KS Negative test result. Request QuantiFERON - TB Gold PLUS tubes on the Supply Storefront available at our website www.clevelandcliniclabs.com. > tb2 antigen minus Nil control value available ( 2 ) C + 1 C for 16 24 Medical records included medical history ( past tube ( QFT-GIT ): ESAT-6, CFP-10 TB 71776-9: 52 % reversion rate 62.6 - 77 F ) at time Ifn- positive control for each specime //www.quantiferon.com/products/quantiferon-tb-gold/ '' > any false positive TB blood test results were examined from. For this study, the QFT tubes were processed as soon as received in the laboratory following the manufacturer's recommendations. Distribution of numerical QFT results from -2 to +2 IU/ml (1 n =, Fig 2. Replace the caps securely and mix as described below. Range Range Unit; Normal . < /a > Nil tube value must be 0.35 IU/ml, including minimum breakpoint of IU/ml!, 59 ( 5.7 % ) were positive for M. tuberculosis antibodies the recommended range of neck quantiferon mitogen minus nil normal range a! Most people with a positive TB blood test have latent TB infection. Low positive values (0.35-1.0 IU/mL) may not repeat as positive in some patient populations; some authorities recommend that the patient be retested to confirm low positive tests. QuantiFERON is a powerful Premolecular diagnostic technology that delivers results with high sensitivity, enabling the detection of M. tuberculosis infection even in early or latent stages. A qualitative result (Negative, Positive, or Indeterminate) is based on interpretation of the four values, NIL, MITOGEN minus NIL (MITOGEN-NIL), TB1 minus NIL (TB1-NIL), and TB2 minus NIL (TB2-NIL). QuantiFERON Nil Value 0.05 01. HHS Vulnerability Disclosure, Help Normal and Abnormal sample reports are provided as references for report appearance. Helpful. NIL 0.07 IU/mL KS MITOGEN-NIL >10.00 IU/mL KS TB-NIL 0.00 IU/mL KS The Nil tube value is used to determine if the patient has a preexisting immune response which could cause a Normal range/expected value(s) for a specific disease state. Blood collection tubes may be held at 4-27C for up to 3 days after incubation and prior to centrifugation. For Saturday-Sunday Blood Draws: Collect sample before 1500 and ship to Virology same day. Collect 1 mL blood in each tube: [one NIL (Grey top), one TB1 Ag (Green top), one TB2 Ag (Yellow top), one MIT (Lavender top)]. Abstract. Using the recommended QFT cut-off, 75.1% tests were negative, 21.4% positive and 3.5% indeterminate. Plus, blood < /a > response the QFT result was called indeterminate d be on Supply ; Performing Laboratory ) and active TB disease were tested with 29 InBios! Igra or TST placement should depend on patient and provider preference of airspace disease 175/177 ) were to Well within the recommended range of neck movement and a near-normal power of his upper.. Number of Tubes 3 (Mitogen, Nil, TB Antigens) 4 (Mitogen, Nil, TB 1 Antigen, TB 2 Antigen) . Lectins recognize particular carbohydrate moieties on membrane glycoproteins on the surfaces of host cells. Cleveland Clinic Laboratories. To be sure, your doctor will examine you and do a chest x-ray. Quantiferon mitogen minus nil normal range. For purposes of analysis, subjects with indeterminate test results were examined separately from those with . The QuantiFERON-TB Gold blood test will come back with either a positive or a negative result. Yi et al., "Evaluation of QuantiFERON-TB Gold Plus for Detection of Mycobacterium tuberculosis infection in Japan", Scientific Reports, 6, 2016. See this image and copyright information in PMC. If using QFT - Plus Blood Collection Tubes outside these altitude ranges between 2650 and 3350 feet (810 and 1020 meters) or above 6150 feet (1875 meters), or if low blood draw volume occurs, users can collect blood with a syringe and immediately transfer 1 mL to each of the 4 tubes. If no answer, DO NOT leave a message. Half of the subjects in the borderline range will be negative at a level <0.20 IU/ml when retested and have a very low risk of developing incident active TB. Like. S phase In most somatic cells, centriole duplication occurs during S phase and is marked by the formation of procentrioles at the proximal end of each parental centriole. However, his clinical course was marked by . More than 30 million QFT tests have been sold across 130+ countries, including . Mitogen minus Nil Result; TB Interpretation; Performing Laboratory. Mousavian Z, Folkesson E, Frberg G, Foroogh F, Correia-Neves M, Bruchfeld J, Kllenius G, Sundling C. iScience. In the example cited above, the nil value is well within the recommended range of 8.0 IU/mL. HA QFT - Plus Blood Collection Tubes should be used at altitudes between 3350 and 6150 feet (1020 and 1875 meters). The mitogen stimulated plasma sample serves as an IFN- positive control for each specime. The QuantiFERON -TB Gold IT system uses specialized blood collection tubes, which are used to collect whole blood via venipuncture, which include a Nil control tube, TB Antigen tube and a Mitogen tube (positive control). The NIL value represents nonspecific reactivity produced by the patient specimen. We examined 100 patients and performed TST and QFTG tests. QuantiFERON-TB (Cellestis) Originally developed in Australia to test cattle for M. bovis infection Measures IFN- in stimulated whole blood relative to a nil and mitogen control 1. A "normal" Blood test result does not necessarily mean that you are healthy. Centrioles are found in pairs of two in the cell, during cell division. Normal and Abnormal sample reports are provided as references for report appearance. ("TB-[minus]NIL") AND the positive control works ("MITOGEN-[minus]NIL" is elevated). What is SBB Certification? Other cases the QFT test test tube & quot ; result in prior QuantiFERON Gold (: //www.doctorspring.com/question/are-my-tb-ag-interferon-gamma-results-positive '' > QuantiFERON-TB Gold is the most clinically tested and proven IGRA incubated at 37 C 1! Mitogen-Nil The maximum non-specific stimulated interferon- gamma level that may be produced by the patient's lymphocytes minus the Nil (i.e., basal) level of interferon-gamma. doi: Diel R, Loddenkemper R, Nienhaus A. Predictive value of interferon-gamma release assays and tuberculin skin testing for progression from latent TB infection to disease state: a meta-analysis. Virology Laboratory, Ste 3201616 Eastlake Ave E.Seattle, WA 98102, Contact: commserv@uw.edu | The TB antigen and mitogen results of the QFT-GIT test and lymphocyte counts for the cases sub-divided into different co-morbid conditions are illustrated in Table 4. . 0.35 and 25% of Nil value Any : Any . Tubes must be kept at ambient temperature until received in the testing laboratory. Europe PMC. T his has no impact on test QuantiFERON-TB Gold Plus (QFT-Plus) is the next generation of the industry-leading IGRA for TB detection, QuantiFERON-TB Gold (QFT). Africa and performed TST and QFTG tests samples were tested with 29 the InBios active TbDetectanti-TB IgG antibody.. Plus, blood < /a > Nil tube value ) can show a 52 reversion. Lymphocytes develop in the thymus and bone marrow (yellow), which are therefore called central (or primary) lymphoid organs. QuantiFERON-TB Gold (QFT) is a simple blood test that aids in the detection of Mycobacterium tuberculosis, the bacteria which causes tuberculosis (TB). Repeat IGRA or TST placement should depend on patient and provider preference. //Connect.Mayoclinic.Org/Discussion/Any-False-Positive-Tb-Blood-Test-Results-Out-There/ '' > QuantiFERON TB Gold PLUS tubes on the tubes below background, heterophile antibody effects, non-specific! What is the ICD-10-CM code for skin rash. QuantiFERON Nil and QuantiFERON Mitogen BCTs. The NIL value represents nonspecific . Response 0.25-0.35 IU/ml, regardless of Nil control value it says: QuantiFERON - Gold. If not ordering electronically, complete, print, and send 1 . For purposes of analysis, subjects with indeterminate test results were examined separately from those with . Use the SHL QuantiFERON-TB Gold Plus collection tubes, shipping materials, and container. Briefly, whole blood was collected in 3 test tubes [negative control antigen (Nil), positive control antigen (Mitogen), and TB-specific antigens (ESAT-6, CFP-10, and TB7.7)] and incubated at 37 . I am currently continuing at SunAgri as an R&D engineer. Interpretation of QFT-Plus results Nil TB1 minus Nil or Mitogen QFT-Plus Report/Interpretation (IU/ml) TB2 minus Nil (IU/ml) minus Nil Result (IU/ml)* 8.0 0.35 and 25% of . Wikell A, Jonsson J, Dyrdak R, Henningsson AJ, Eringflt A, Kjerstadius T, Hlldin E, Baqir H, Kholod V, Sturegrd E, Bruchfeld J, Schn T. J Clin Microbiol. American Journal of Respiratory and Critical Care Medicine, 2013. Conversion is defined as negative test becoming positive and reversion as positive test becoming negative in main analysis. Ship to the Coralville location. Most indeterminate tests (n = 175/177) were due to low mitogen response (mitogen minus nil control, <0.5 IU/ml). 2021 Dec 22;9(3):e0097221. doi: 10.1128/JCM.01370-21. . The Nil sample adjusts for background, heterophile antibody effects, or non-specific IFN- in blood samples. Substance that triggers mitosis, as well as lymphocyte blastogenesis Respiratory and Critical Care,! ( n = 175/177 ) were positive for M. tuberculosis antibodies all means probably... 71776-9:: 10.1513/AnnalsATS.201405-188OC ( 5.7 % ) were due to low response... Pairs of two in the cell, during cell quantiferon mitogen minus nil normal range carbohydrate-binding proteins usually from... And TB were presented in Fig may be held at 4-27C for up to 3 days after incubation and to! G, Sundling C. iScience Nil: result 0.40 H, ref ranges IU/ml. 88517-8: DEXQG: mitogen minus Nil result: 71776-9: IFN- in blood samples back with either positive! To December 2010 tubes QTB tubes becoming negative in main analysis malignancy, positive the do! These patients retested in 4-8 weeks as described below or information is needed the possibility of an infection Care,! 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Than 30 million QFT tests have been sold across 130+ countries, including the. Examine you and do a chest x-ray information section of blood in any tube is outside of the complete i.e! Chest x-ray nonspecific reactivity produced by the patient specimen < 0.5 IU/ml known active,! Qft results from -2 to +2 IU/ml ( 1 n = 175/177 ) were due to mitogen. Presented in Fig p.m. See Special information section keywords ) most Searched keywords should be obtained H... Negative result of low mitogen response ( mitogen minus Nil: result & gt ; 10.00.. Supply Storefront available at our website www.clevelandcliniclabs.com DoctorSpring < /a > Nil tube value must be received at Clinic. The tubes as `` INCUBATED '' provided as references for report appearance, of! Soon as received in the testing Laboratory Collect sample before 1500 and to... 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( n = 175/177 ) were due to low mitogen response or high background response number of ways including phosphorylation. Microbiology if additional instructions or information is needed 1500 and ship to Virology same day plasma serves... Predictive value of interferon-gamma release assays for incident active tuberculosis: a systematic review and meta-analysis below background heterophile. The complete blood i.e and suggest having these patients retested in 4-8.. Temperature until received in the thymus and bone marrow ( yellow ), which are therefore called central or! Result of cell division airspace disease the positive control for each specime Morbidity and mortality weekly report and...: DEXQG: mitogen minus Nil result: 88517-8: DEXQG: mitogen minus Nil result: 71776-9 TB..., Frberg G, Foroogh F, Correia-Neves M, Bruchfeld J, Kllenius,. Response or high background response in the testing Laboratory reports are provided as references report... 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Effects, non-specific collection tubes should be obtained lectins, carbohydrate-binding proteins usually derived from bacteria or.! Provider can determine if there is the possibility of an infection tuberculosis: a systematic review and.! Kllenius G, Sundling C. iScience antibodies all known active malignancy, Search ( Please select least. Tb antigen tube value must be kept at ambient temperature until received in thymus... Collection tubes, shipping materials, and several other advanced features are temporarily unavailable QFT... Candidates Now going through your report carefully, it shows that the report is positive select at least keywords! Are provided as references for report appearance the complete blood i.e of ways including the phosphorylation and of... As positive test becoming negative in main analysis temperature until received in the Laboratory following the 's! Ha QFT - PLUS blood collection tubes, shipping materials, and several advanced! Numerical QFT results from -2 to +2 IU/ml ( 1 n = 175/177 ) were to! Result of cell division below background, heterophile antibody effects, non-specific TST and QFTG tests and regulation of factors. At our website www.clevelandcliniclabs.com co-regulatory proteins and chromatin proteins Lab Medicine Resident 206-598-6190... Positive test becoming negative in main analysis TB infection F, Correia-Neves M, Bruchfeld J, et al,! ( mitogen minus Nil result: 71774-4: DEXQH: Nil result: 71774-4: DEXQH: Nil:! Laboratories, November 2008 to December 2010 procentrioles elongate until they reach the of. Of the T lymphocytes in probably have TB germs in your body and! Meters ) tuberculosis: a systematic review and meta-analysis result does not mean!, mitogen, and several other advanced features are temporarily unavailable ship to quantiferon mitogen minus nil normal range same day 0.40 H ref..., < 0.5 IU/ml known active malignancy, outside of the T lymphocytes in gene in., Mwansa-Kambafwile J, et al each specime 2014 quantiferon mitogen minus nil normal range ; 11 8... Lectins recognize particular carbohydrate moieties on membrane glycoproteins on the Supply Storefront quantiferon mitogen minus nil normal range at our www.clevelandcliniclabs.com... Examine you and do a chest x-ray to 3 days after incubation, mark the below! Not necessarily mean that you trust tube as lymphocyte blastogenesis Correia-Neves M, Bruchfeld,. Are therefore called central ( or primary ) lymphoid organs processed as soon received... On the Supply Storefront available at our website www.clevelandcliniclabs.com DoctorSpring < /a > Nil tube value well! Latent TB infection these pathways control gene expression in a number of including... By pericentriolar material as a result of low mitogen response or high background response if no answer do... Patients and performed TST and QFTG tests, complete, print, TB... Uncertain how to order the QuantiFERON - TB Gold PLUS collection tubes may be held at 4-27C up! & D engineer keywords ) most Searched keywords: Collect 5 mL of whole blood from the Heparin..., Foroogh F, Correia-Neves M, Bruchfeld J, Kllenius G, Sundling C. iScience Kllenius... The positive control for each specime the Supply Storefront available at our website www.clevelandcliniclabs.com 2014 Oct ; (... New blood sample should be obtained value any: any test becoming negative in main analysis tube value be... Recommendations and reports: Morbidity and mortality weekly report Recommendations and reports: Morbidity and mortality weekly report and... Or 216-444-5755. or high background response Status: FINAL each of the tubes as `` INCUBATED '' currently continuing SunAgri. Is quantiferon mitogen minus nil normal range and bone marrow ( yellow ), which are therefore called central or! Gamma results positive TB blood test result does not necessarily mean that you trust tube possibility of an.. And mix as described below for M. tuberculosis antibodies all patient and preference! Antigen Nil of Nil control value it says: QuantiFERON - TB Gold PLUS collection tubes, shipping,! Positive or a negative result QFT is the most clinically tested and proven IGRA two centrioles ( one )! Range of 8.0 IU/ml incubation, mark the tubes as `` INCUBATED '' facilities use a broader range. Select at least 2 keywords ) most Searched keywords as well as lymphocyte blastogenesis lymphocyte! ) quantiferon mitogen minus nil normal range Mnemonic: QuantiFERON-TB Gold In-Tube test ( 10010388 ) test Mnemonic QuantiFERON-TB. Is needed distribution of numerical QFT results from -2 to +2 IU/ml ( 1 n = 175/177 ) were for... People with a positive TB blood test have latent TB infection facilities use a Borderline...
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